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2 edition of Effects of freezing rate of ram semen on subsequent fertility in vivo and in vitro found in the catalog.

Effects of freezing rate of ram semen on subsequent fertility in vivo and in vitro

Garret P. Byrne

Effects of freezing rate of ram semen on subsequent fertility in vivo and in vitro

by Garret P. Byrne

  • 351 Want to read
  • 8 Currently reading

Published by University College Dublin in Dublin .
Written in English

    Subjects:
  • Frozen semen.,
  • Rams -- Spermatozoa.,
  • Rams -- Fertility.,
  • Sheep -- Reproduction.

  • Edition Notes

    Statementby Garret P. Byrne.
    ContributionsUniversity College Dublin. Department of Animal Science and Production.
    The Physical Object
    Paginationvi, 143p. :
    Number of Pages143
    ID Numbers
    Open LibraryOL18221752M

    In relation to live cells in total and sperm motility in semen of six males, higher values (Psemen frozen in straws. However, taking into consideration the percentage of live normal sperm, a significant (Peffect of freezing method was found only for two capercaillies (♂ no. 58 and no. 49) (Table 3). The use of semen freezing therefore makes it possible for men to preserve their fertility or undergo life saving medical treatment, with the chance of future fertility success. There is also mounting evidence that semen quality is reduced in men from the age of 40 and as such, it may be considered useful to freeze semen early in a man’s.

    However, even for cattle, the current methods for semen freezing are suboptimal. Survival of potentially fertile spermatozoa is low and their subsequent fertility often is under 50% (Rodríguez-Martinez, b). Porcine, equine, canine, or ovine species seem condemned to the use of extended chilled liquid semen for AI since development of. Garret P. Byrne has written: 'Effects of freezing rate of ram semen on subsequent fertility in vivo and in vitro' -- subject(s): Rams, Sheep, Reproduction, Spermatozoa, Fertility, Frozen semen.

    Such an extender is commercially available for bull semen (Bioexcell), IMV, L'Aigle, France), and it has previously been tested in vitro for freezing ram semen, with satisfactory results. The aim of the present study was to compare the fertility results of ewes in Uruguay, after cervical insemination with ram semen that was frozen in Bioexcell. In vitro effects of nonylphenol on motility, mitochondrial, acrosomal and chromatin integrity of ram and boar spermatozoa. The effects of cooling rates and type of freezing extenders on cryosurvival of rat sperm. Cryobiology, Gene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocysts. Molecular.


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Effects of freezing rate of ram semen on subsequent fertility in vivo and in vitro by Garret P. Byrne Download PDF EPUB FB2

The objectives of the present study were to examine how freezing rate through this critical temperature zone affected the fertility of spermatozoa as assessed in vivo and in vitro. Semen from six adult rams was frozen at two different rates ("fast": 5 degrees C/min from +5 to degrees C; "slow": degrees C/min from +5 to degrees C).Cited by:   Byrne GP, Lonergan P, Wade M, Duffy P, Donovan A, Hanrahan JP, Boland MP: Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro.

Anim Reprod Sci./S(00) PubMed CAS Article Google ScholarCited by:   Moreover, Lymberopoulos et al. () correlated ram's age with semen quality and in vivo fertility, using a homologous gametes binding test, the hemizona binding assay.

Concluding remarks. Fertilization is a complex process with many different sequential events. Sperm tests usually assess only one or a few of these by:   The effect of increasing trehalose concentrations added to the isotonic diluent on the sperm motility after freezing and thawing is shown in Fig.

ose mOsm conferred the highest sperm motility values, with an improvement of a 35% over the isotonic diluent (Peffect on motility Cited by:   select article Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro.

Research article Full text access Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro. G.P Byrne, P Lonergan, M Wade, P Duffy, M.P Boland.

Pages Download PDF. Experiment 5 aimed to test the in vivo fertility of There are no studies on the effects of freezing and thawing rates on ejaculated alpaca spermatozoa.

Frozen storage of ram semen. It has long been known that cooling bovine, ovine, caprine, swine stallion semen too rapidly between 30°C and 0°C induces a lethal stress in sperms proportional to cooling rate and temperature range (Watson, ).

Eight times more cryopreserved sperms are required as compared to fresh sperms to achieve fertilization in vivo (Shannon and Vishwanath, ).

The post-thaw survival and fertility of ram spermatozoa frozen in pellets, and ml PVC straws, and ml minitubes were examined. In 5 experiments, a freezing height of 6 cm above the level of liquid nitrogen was optimal for and ml straws, whereas 4.

Semen collection and sperm evaluation and addition of preservatives for increasing storage of sperm are essential for successful artificial insemination. This study was conducted on 4 rams (2 Ghezel Merinos and 2 Merinos Moghani) rams to evaluate the effect of 2 liquid diluters (1-egg yolkcitrate, 2milk) in Khalat poshan research station.

Byrne GP, Lonergan P, Wade M, Duffy P, Donavan A, Hanrahan JP et al () Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro. Anim Reprod Sci – doi: /S(00) The aim of this study was to determine the effects of the administration time of misoprostol (11 h (Miso11) and 6 h (Miso6) before artificial insemination) on fertility rates in Kivircik ewes.

Abstract Research up to is reviewed. Various methods for processing, freezing and thawing of semen have been elaborated and their effect on survival and fertility of spermatozoa have been examined in vitro or in fertility tests. Diluents initially used for freezing bull semen had their limitations and were modified or replaced by new media.

The freezing diluents investigated included. The effect of straw size, freezing rate and thawing rate upon post-thaw In vitro and in vivo evaluation of ram sperm frozen in tris egg-yolk and supplemented with Ruiz S.

Analysis of in vitro fertilizing capacity to evaluate the freezing procedures of boar semen and to predict the subsequent fertility. Reprod Domest Anim ; on the cooling and freezing rates (Fisher and Fairfull, ), diluents composition (Pontbriand et al., ) and method of adding the glycerol (Colas, ).

However, all new methods for processing ram semen need to be tested before practical application in the field. In vitro. Semen cryopreservation (commonly called sperm banking or sperm freezing) is a procedure to preserve sperm can be used successfully indefinitely after human sperm, the longest reported successful storage is 24 years.

It can be used for sperm donation where the recipient wants the treatment in a different time or place, or as a means of preserving fertility for. 2. Oxidative Stress. One of the most important factors contributing to poor quality semen has been reported to be oxidative stress [].Oxidative stress is a condition associated with an increased rate of cellular damage induced by oxygen and oxygen derived oxidants commonly known as ROS [].Uncontrolled production of ROS that exceeds the antioxidant capacity of the seminal plasma leads.

The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, | Explore the latest full-text research PDFs. Explore the latest full-text research PDFs, articles, conference papers, preprints and more on OOCYTE VITRIFICATION.

Find methods information, sources, references or conduct a literature review on. Specific features of in vivo and in vitro sperm storage in birds - Volume 1 Issue 10 - E.

Blesbois, J. Brillard Effect on fertility of storing turkey semen for 24 hours at 10°C in fluids of different pH. Effects of the freezing rate on viability and fertility of frozenthawed fowl spermatozoa. In previous studies, we showed that the loss of sperm fertility following in vitro exposure to BPA ( µM) was mediated by decreased motility and premature triggering of.

It seldom recurs and the pregnancy rate in subsequent ICSI cycles is normal. Infection of culture dishes: very occasionally culture media may become contaminated with bacteria from the semen or from the vagina during egg collection which leads to the embryos dying.

There are various strategies to minimise the risk in subsequent cycles.Many IVF clinics freeze their embryos at the blastocyst stage. Sperm Freezing. Sperm freezing is also known as cryopreservation or semen banking is a technique of preserving sperms by freezing and storing in liquid nitrogen at the temperature of ⁰C that is used on a later date for fertilizing an egg.

Sperm freezing is useful if.R. B. Buckland, “The activity of six enzymes of chicken seminal plasma and sperm. 1. Effect of in vitro storage and full sib families on enzyme activity and fertility,” Poultry science, vol.

50, no. 6, pp. –, View at: Google Scholar.